Codon Optimizer

Optimized Codons → sequence is optimized based on the best motifs in different regions, balancing codon usage, wobble, and motif constraints.

C wobbling → amino acids are encoded using codons that prefer C at the wobble position (C > G > A/T). For serine (S) the priority is TCC, for proline (P) the priority is CCC. If more than four Cs occur in a row, codons with G at the wobble position are chosen to break the run.